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They had free access to water and were fed a standard diet according to their weight.Pens contained wood shavings, straw and toys for environmental enrichment.
Formulations with 1-methyl-niacinamide, an analogue of niacinamide, were also administered.
Animal experiments were carried out in accordance with the Danish Act on Experiments on Animals, the Appendix A of ETS 123 and EU Directive 2010/63.
A procedure project license was issued by the national authority.
Tissue samples measuring ~2 x 2 cm were excised at 0 min (acute), 2, 5, 15 or 60 min after injection and immediately frozen in 2-methyl-butane.
To evaluate whether niacinamide and 1-methyl-niacinamide were entirely confined within the excised tissue, 1 cm thick sections along the four edges of the 0 min (acute) sample were also excised.
Limiting excessive postprandial glucose (PPG) excursions is a major challenge in the treatment of diabetes.
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