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25-Dec-2019 07:45
Researchers are starting to take advantage of the technology by combining multiple end points in a single assay.At the same time, advances in RNA interference (RNAi) reagents have allowed workers to knock down genes on a large scale."Initially, we had a variety of plate readers and confocal microscopy to validate targets one at a time, but it was rather laborious.The idea was to develop a uniform platform to handle multiple targets simultaneously.
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The screens are constantly growing in size, and in the next few years, Wardwell-Swanson anticipates genome-wide screens of tens of thousands of genes, which may require higher throughput, second-generation instruments.
A particular software application might evaluate one or more of a half-dozen major classes of cellular change, from identifying spots to tracking changes in morphology, translocation or intensity.
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